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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 13, Number 3
BKCSDE 13(3)
March 20, 1992 

 
Title
Effect of SDS on Retention of Nucleic Acid Components in High-Performance Liquid Chromatography
Author
Yong-Nam Kim*, Kyeung-Soo Choi, Dai-Woon Lee, Phyllis R. Brown
Keywords
Abstract
The effect of the addition of sodium dodecyl sulfate (SDS) to a buffered mobile phase (pH 3.4) on the retention of nucleotides, nucleosides and bases was investigated with a polyvinyl alcohol (PVA) column. Depending on the concentration of SDS, two different trends in the retention of nucleosides and bases containing an NH2 group were observed. If the concentration of SDS was less than 5.5 mM, the retention of compounds containing an NH2 group increased as the concentration of SDS in the mobile phase increased. In contrast, if the concentration was greater than 5.5 mM, the retention of compounds containing an NH2 group decreased. Thus, the SDS acted as an ion-pairing reagent at lower concentration but formed micelles at higher concentrations. The retention behavior of the nucleosides and bases in the presence of a micellar concentration of SDS in the mobile phase on the PVA column was compared to the retention behavior on other types of columns.
Page
240 - 245
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