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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 4, Number 2
February 20, 1983 

Enzymatic Synthesis of Cephaloglycin
Doo Hyun Nam, Heon Soo Sohn, Dewey D. Y. Ryu
Cephaloglycin was synthesized directly from D-α -phenylglycine methyl ester and 7-aminocephalosporanic acid using whole cell enzyme of Xanthomonas citri (IFO 3835). Some optimal conditions for cephaloglycin synthesis were investigated, and yield improvements for its production by several methods were attempted. Using the whole cell enzyme system, the reaction kinetic model for cephaloglycin synthesis is proposed, and the kinetic constants for D-α -phenylglycine methyl ester hydrolysis, cephaloglycin synthesis, and cephaloglycin hydrolysis were determined. The Km values of D-α -phenylglycine methyl ester, 7-aminocephalosporanic acid, and cephaloglycin were 11 mM, 24 mM, and 167 mM, and Ki value of D-α-phenylglycine was 15 mM, respectively. The pattern of product inhibition was found to be competitive one.
72 - 76
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