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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 35, Number 8
BKCSDE 35(8)
August 20, 2014 

Synthesis, Characterization and DNA Interaction Studies of (N,N'-Bis(5-phenylazosalicylaldehyde)-ethylenediamine) Cobalt(II) Complex
Nasrin Sohrabi*, Nahid Rasouli, Mehdi Kamkar
Azo schiff base, Calf thymus DNA, UV-vis spectroscopy, Viscosity, Thermal denaturation
In the present study, at first, azo Schiff base ligand of (N,N'-bis(5-phenylazosalicylaldehyde)-ethylenediamine) (H2L) has been synthesized by condensation reaction of 5-phenylazosalicylaldehyde and ethylenediamine in 2:1 molar ratio, respectively. Then, its cobalt complex (CoL) was synthesized by reaction of Co(OAc)2·4H2O with ligand (H2L) in 1:1 molar ratio in ethanol solvent. This ligand and its cobalt complex containing azo functional groups were characterized using elemental analysis, 1H-NMR, UV-vis and IR spectroscopies. Subsequently, the interaction between native calf thymus deoxyribonucleic acid (ct-DNA) and CoL complex was investigated in 10 mM Tris/HCl buffer solution, pH = 7 using UV-vis absorption, thermal denaturation technique and viscosity measurements. From spectrophotometric titration experiments, the binding constant of CoL complex with ct-DNA was found to be (2.4 ± 0.2) × 104 M−1. The thermodynamic parameters were calculated by van’t Hoff equation.The enthalpy and entropy changes were 5753.94 ± 172.66 kcal/mol and 43.93 ± 1.18 cal/mol·K at 25 °C, respectively. Thermal denaturation experiments represent the increasing of melting temperature of ct-DNA (about 0.93 °C) due to binding of CoL complex. The results indicate that the process is entropy- driven and suggest that hydrophobic interactions are the main driving force for the complex formation.
2523 - 2528
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