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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 34, Number 9
BKCSDE 34(9)
September 20, 2013 

Liquid Chromatography-Tandem Mass Spectrometric Determination of Geniposide in Rat Plasma and its Pharmacokinetic Application
Ming Ji Jin, In Sook Kim, Dong-Hyun Kim, Hye Hyun Yoo*
Geniposide, LC-MS/MS, Pharmacokinetics, Rats
Geniposide is a biologically active ingredient of gardenia fruit. A liquid chromatography-tandem mass spectrometric method was developed and validated for the determination of geniposide in rat plasma. The plasma samples were pretreated by solid-phase extraction and introduced into a BDS Hypersil C18 column (50 × 2.1 mm, 5 μm) for chromatographic separation. The mobile phase consisted of 0.1% formic acid and 0.1% formic acid in acetonitrile, and gradient elution was performed at a flow rate of 0.25 mL/min. For mass spectrometric detection, multiple reaction monitoring was performed via an electrospray ionization source in positive mode. The calibration curve for geniposide was linear (r2 = 0.997) in the concentration range of 0.005- 1 μg/mL. The intra- and inter-day accuracies and precisions fulfilled the required criteria (± 15%). The developed method was subsequently used for pharmacokinetic analysis of geniposide after oral administration to rats at a dose of 50 mg/kg. The mean maximum plasma concentration of geniposide was 0.68 ± 0.29 μg/mL at 0.44 ± 0.13 h, and the mean area under the plasma concentration versus time curve was 1.46 μg·h/mL.
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