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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 33, Number 12
BKCSDE 33(12)
December 20, 2012 

Microfluidic Image Cytometry (μFIC) Assessments of Silver Nanoparticle Cytotoxicity
Jonghoon Park, Tae Hyun Yoon*
Microfluidic image cytometry, Microfluidic device, Cytotoxicity assessment, Ag nanoparticles, Nanotoxicity
Cytotoxicity assessment of silver nanoparticles (AgNPs) was performed using MTT-based microfluidic image cytometry (μFIC). The LC50 value of HeLa cells exposed to AgNPs in the microfluidic device was estimated as 46.7 mg/L, which is similar to that estimated by MTT-based IC for cells cultured in a 96 well plate (49.9 mg/ L). These results confirm that the μFIC approach can produce cytotoxicity data that is reasonably well-matched with that of the conventional 96 well plate system with much higher efficiency. This μFIC method provides many benefits including ease of use and low cost, and is a more rapid in vitro cell based assay for AgNPs. This may aid in speeding up data acquisition in the field of nanosafety and make a significant contribution to the quantitative understanding of nanoproperty- toxicity relationships.
4023 - 4027
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