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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 33, Number 2
BKCSDE 33(2)
February 20, 2012 

Solution Structure of LXXLL-related Cofactor Peptide of Orphan Nuclear Receptor FTZ-F1
Ji-Hye Yun, Chul-Jin Lee, Jin-Won Jung, Weontae Lee *
Fushi tarazu, FTZ-F1, Cofactor, NMR, LXXLL motif
Functional interaction between Drosophila orphan receptor FTZ-F1 (NR5A3) and a segmentation gene product fushi tarazu (FTZ) is crucial for regulating genes related to define the identities of alternate segmental regions in the Drosophila embryo. FTZ binding to the ligand-binding domain (LBD) of FTZ-F1 is of essence in activating its transcription process. We determined solution structures of the cofactor peptide (FTZPEP) derived from FTZ by NMR spectroscopy. The cofactor peptide showed a nascent helical conformation in aqueous solution, however, the helicity was increased in the presence of TFE. Furthermore, FTZPEP formed α- helical conformation upon FTZ-F1 binding, which provides a receptor bound structure of FTZPEP. The solution structure of FTZPEP in the presence of FTZ-F1 displays a long stretch of the α-helix with a bend in the middle of helix.
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