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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 33, Number 1
BKCSDE 33(1)
January 20, 2012 

Interaction of Apidaecin Ib with Phospholipid Bilayers and its Edwardsiella Species-specific Antimicrobial Activity
Jung-Kil Seo, Hye-Jin Go, Ho Sung Moon, Min Jeong Lee, Yong-Ki Hong, Hyun Do Jeong, Bo-Hye Nam, Tae Hyun Park, Nam Gyu Park*
Apidaecin Ib, Edwardsiella species, Alternatives, Fish pathogenic bacteria
Apidaecin Ib had strong antimicrobial activity against several tested Gram-negative bacteria including Escherichia coli, Enterobacter cloacae, and Shigella flexneri (MECs; 0.3-1.5 μg/mL), but showed no activity against all the tested Gram-positive bacteria including Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus and one yeast, Candida albicans (MECs; >125 μg/mL). Interestingly, this peptide showed potent antibacterial activity only against Edwardsiella species (MECs; 0.6-3.6 μg/mL) among the tested fish pathogenic bacteria through a bacteriostatic process and showed no significant hemolytic activity. Apidaecin Ib took an unordered structure in all environments and also had very weak membrane perturbation activity even at 25 μM. Anti-Edwardsiella activity of apidaecin Ib is stronger than those of other antimicrobial polypeptides or antibiotics, but its activity is salt-sensitive. These results suggest that apidaecin Ib has Edwardsiella speciesspecific antibacterial activity and could be applied as new preventive or control additives for Edwardsiella species infection in freshwater fish aquaculture.
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