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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 32, Number 12
BKCSDE 32(12)
December 20, 2011

Title	Characterization of Two Ricin Isoforms by Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis and Capillary Isoelectric Focusing
Author	Dong Hee Na, Eun Ji Park, Myung Sun Kim, Cheong Kwan Cho, Byung Ho Woo, Hye Suk Lee, Kang Choon Lee,
Keywords	Ricin, Capillary electrophoresis, Capillary isoelectric focusing, Sodium dodecyl sulfate-capillary gel electrophoresis
Abstract	Sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) and capillary isoelectric focusing (CIEF) were employed to characterize and compare ricin E purified from the small grain seeds of Ricinus communis with ricin D isoform. During the purification of ricin E using ion-exchange and size-exclusion chromatography, SDS-CGE was found to be useful for monitoring the efficiencies of purification steps. SDS-CGE showed that the molecular size of ricin E was not significantly different from that of ricin D, which was confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. CIEF was useful for discriminating ricin E from ricin D based on their isoelectric points (pI). The pI values of ricin E and D were 8.6-8.8 and 7.0-7.4, respectively. This study demonstrates the usefulness of SDS-CGE and CIEF for the characterization of ricin toxins.
Page	4253 - 4257
Full Text	PDF

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