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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 32, Number 8
BKCSDE 32(8)
August 20, 2011 

Energy Transfer from Ethidium to Cationic Porphyrins Mediated by DNA and Synthetic Polynucleotides at Low Binding Densities
Jin-A Jung, Sun Hee Jeon, Sung Wook Han, Gil Jun Lee, In-Ho Bae, Seog K. Kim*
Energy transfer, Porphyrin, Ethidium, DNA, Polynucleotide, Fluorescence
The fluorescence of ethidium bound to DNA, poly[d(A-T)2], and poly[d(G-C)2] at a [ethidium]/[DNA] ratio of 0.005 was quenched by porphyrins when both ethidium and the porphyrins simultaneously bound to the same polynucleotide. The quenching was tested using the “inner sphere” and the “Förster resonance energy transfer” (FRET) models, with the latter found to contribute, at least in part, to the quenching. Meso-tetrakis(Nmethylpyridinium- 4-yl)porphyrin (TMPyP) exhibited a higher quenching and FRET efficiency than cis-bis(Nmethylpyridinium- 4-yl)porphyrin (BMPyP) for all of the tested DNA and polynucleotides, demonstrating that energy transfer efficiency is affected by the number of positive charges of porphyrins.
2599 - 2606
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