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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 32, Number 4
BKCSDE 32(4)
April 20, 2011

Title	An Electrochemical Enzyme Immunochip Based on Capacitance Measurement for the Detection of IgG
Author	Seung Jae Yi, Ji Hye Choi, Hwa Jung Kim, Seung Cheol Chang,, Deog Su Park, Kyung Chun Kim, Chulhun L. Chang,
Keywords	Immunochip, Enzyme immunoassay, Capacitance, Impedometric, Interdigitated electrode
Abstract	This study describes the development of an electrochemical array immunochip for the detection of IgG. Interdigitated immunochip platforms were fabricated by sputtering gold on a glass wafer by using MEMS process and then were coated with Eudragit S100, an enteric polymer, forming an insulating layer over the working area of immunochips. The breakdown of the polymer layer was exemplified by the catalytic action of urease which, in the presence of urea, caused an alkaline pH change. This subsequently caused an increase of the double layer capacitance of the underlying electrode. Used in conjunction with a competitive immunoassay format, this allowed the ratio of initial to final electrode capacitance to be directly linked with the concentration of analyte, i.e. IgG. Responses to IgG could be detected at IgG concentration as low as 250 ngmL−1 and showed good linearity up to IgG concentration as high as 20 μgmL−1.
Page	1298 - 1302
Full Text	PDF

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