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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 32, Number 3
BKCSDE 32(3)
March 20, 2011

Title	Mechanism of DNA Cleavage Induced by Fe²⁺ Autoxidation
Author	Jong Moon Kim, Seog K. Kim *
Keywords	Iron autoxidation, Fenton reaction, Reactive oxygen species, Hydroxyl radical
Abstract	This work investigated the difference between Fe²⁺ autoxidation-induced and Fenton-type cleavage of pBR322 plasmid DNA. ^•OH generation reactions in the absence and presence of H₂O₂ under various conditions were also investigated. Although both the Fe²⁺ autoxidation and Fenton-type reactions showed DNA cleavage and ^•OH generation, there were significant differences in their efficiencies and reaction rates. The rate and efficiency of the cleavage reaction were higher in the absence of 1.0 mM of H₂O₂ than in its presence in 20 mM phosphate buffer. In contrast, the ^•OH generation reaction was more prominent in the presence of H₂O₂ and showed a pH-independent, fast initial reaction rate, but the rate was decreased in the absence of H₂O₂ at across the entire tested pH range. Studies using radical scavengers on DNA cleavage and ^•OH generation reactions in both the absence and presence of H₂O₂ confirmed that both reactions spontaneously involved the active oxygen species ^•OH, O₂ ^•−, ¹O₂ and H₂O₂, indicating that a similar process may participate in both reactions. Based on the above observations, a new mechanism for the Fe²⁺ autoxidation-induced DNA cleavage reaction is proposed.
Page	964 - 972
Full Text	PDF

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