Current time in Korea 04:25 Sep 21 (Fri) Year 2018 KCS KCS Publications
KCS Publications
My Journal Log In Register
HOME > Search > Browsing(BKCS) > Archives

Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 31, Number 6
BKCSDE 31(6)
June 20, 2010 

Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles
Yeon Ji Lee, Jong Moon Park, Jiyoung Huh, Minsik Kim, Je Sun Lee, Arudra Palani, Kwangyeol Lee*, Sang Won Lee*
Proteomics, Glutathione, Superparamagnetic, Nanoparticle, Separation
The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathionemodified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/ MS/MS experiments.
1568 - 1572
Full Text