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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 31, Number 6
BKCSDE 31(6)
June 20, 2010 

Electronic Detection of Biomarkers by Si Field-Effect Transistor from Undiluted Sample Solutions with High Ionic Strengths
Chil Seong Ah*, Ansoon Kim, Wan Joong Kim, Chan Woo Park, Chang Geun Ahn, Jong Heon Yang, In Bok Baek, Tae Youb Kim, Gun Yong Sung*
Electronic detection, PSA, Si field effect transistor (FET), Ionic strength, Real-time
In this study, we have developed a new detection method using Si field effect transistor (FET)-type biosensors, which enables the direct monitoring of antigen-antibody binding within very high-ionic-strength solutions such as 1×PBS and human serum. In the new method, as no additional dilution or desalting processes are required, the FET-type biosensors can be more suitable for ultrasensitive and real-time analysis of raw sample solutions. The new detection scheme is based on the observation that the strength of antigen-antibody-specific binding is significantly influenced by the ionic strength of the reaction solutions. For a prostate specific antigen (PSA), in some conditions, the binding reaction between PSA and anti-PSA in a low-ionic strength reaction solution such as 10 μM phosphate buffer is weak (reversible), while that in high-ionic strength reaction solutions such as 1×PBS or human serum is strong.
1561 - 1567
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