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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 31, Number 5
BKCSDE 31(5)
May 20, 2010 

Bioanalytical Application of SERS Immunoassay for Detection of Prostate-Specific Antigen
Kyung Jin Yoon, Hyeong Kuyn Seo, Hoon Hwang, Dongjin Pyo, In Yong Eom, Jong Hoon Hahn, Young Mee Jung*
Surface-enhanced Raman scattering (SERS), Immunoassay, Prostate-specific antigen (PSA), Raman reporter, Gold nanopaticles
We demonstrate the possible application of the sandwich type surface-enhanced Raman scattering (SERS) immunoassay using antigen-antibody binding for detection of prostate-specific antigen (PSA) in cancer cells. In this sandwich type of SERS immunoassay, to capture antigens onto the immobilized layer of antibodies on the gold substrate we prepared the monolayer of gold nanoparticles on the APTMS-derivatized surface of a glass slide by using the SAM technique. This sandwich type of SERS immunoassay in which antigens on the substrate specifically capture antibodies on a Raman reporter (DSNB coated gold nanoparticles with R6G) could successfully detect PSA at low levels. A strong SERS spectrum of Raman reporter was observed only with a substrate in which PSA is present.
1215 - 1218
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