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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 31, Number 5
BKCSDE 31(5)
May 20, 2010 

Construction of 1H-15N Double Resonance Solid-State NMR Probe
for Membrane Proteins in Aligned Bicelles
Tae Joon Park, Ji Sun Kim, Seung Hoon Um, Yongae Kim*
Solid-state NMR, Home-built probe, Membrane proteins, Bicelles, Bilayers
1H-15N heteronuclear dipolar coupling solid-state NMR experiments on lipid bilayer or bicelle samples are very useful for the structural studies of membrane proteins. However, to study these biological samples using solid-state NMR, a specific probe with high efficiency and high capability is required. In this paper, we describe the optimized design, construction, and efficiency of a 400 MHz wide-bore 1H-15N solid-state NMR probe with 5-mm solenoidal rf coil for high power, multi-pulse sequence experiments, such as 2D PISEMA or 2D SAMMY.
1187 - 1191
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