Current time in Korea 16:49 Nov 25 (Sat) Year 2017 KCS KCS Publications
KCS Publications
My Journal Log In Register
HOME > Search > Browsing(BKCS) > Archives

Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 30, Number 8
BKCSDE 30(8)
August 20, 2009 

 
Title
ATP Hydrolysis Analysis of Severe Acute Respiratory Syndrome (SARS) Coronavirus Helicase
Author
Na Ra Lee, A Ram Lee, Bokhui Lee, Dong Eun Kim*, Yong Joo Jeong*
Keywords
Severe acute respiratory syndrome coronavirus helicase, ATP hydrolysis, Single-stranded nucleic acids, pH-Dependence profile, Divalent cations
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) helicase separates the double-stranded nucleic acids using the energy from ATP hydrolysis. We have measured ATPase activity of SARS-CoV helicase in the presence of various types of nucleic acids. Steady state ATPase analysis showed that poly(U) has two-times higher turnover number than poly(C) with lower Michaelis constant. When M13 single-stranded DNA is used as substrate, the Michaelis constant was about twenty-times lower than poly(U), whereas turnover numbers were similar. However, stimulation of ATPase activity was not observed in the presence of double-stranded DNA. pH dependent profiles of ATP hydrolysis with the helicase showed that the optimal ATPase activities were in a range of pH 6.2 ~ 6.6. In addition, ATP hydrolysis activity assays performed in the presence of various divalent cations exhibited that Mg2+ stimulated the ATPase activity with the highest rate and Mn2+ with about 40% rate as compared to the Mg2+.
Page
1724 - 1728
Full Text
PDF