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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 30, Number 7
BKCSDE 30(7)
July 20, 2009

Title	Determination of Adrenosterone and its Metabolites in Human Urine by LC/APCI/MS and GC/MS
Author	Eunjung Han, Okkyoung Yim, Sun young Beak, Jaeyeon Chung, Ji hye Lee, Jungahn Kim, Yunje Kim*
Keywords	Adrenosterone, Metabolites, LC/APCI/MS, GC/MS
Abstract	This study was done for the determination and excretion profile of adrenosterone and its metabolites in human urine using both liquid chromatography with atmospheric pressure chemical ionization mass spectrometry and gas chromatography with mass spectrometry. Adrenosterone and its two metabolites were detected in human urine after administration a healthy volunteer with 75 mg of adrenosterone. We found that adrenosterone-M1 (C₁₉H₂₆O₃) was a reduction and adrenosterone-M2 (C₁₉H₂₆O₄) was a hydroxylation at C-ring, which did not know the exact position of the C-ring. The adrenosterone parent was detected by GC/TOF-MS, but not detected by LC/APCI/MS because of low intensity. Adrenosterone and its two metabolites were excreted as their glucuronided fractions. The recovery of this method ranged from 100.7 to 118.4% and the reproducibility and accuracy test were 85.5 to 112.0% and 1.1 to 8.4%, respectively. The excretion studies showed that adrenosterone and its metabolites were detectable in human urine during a 48 h period after oral administration, with maximum level of excretion at 4.1 h. The glucuro-/sulfaconjugated ratio of adrenosterone, M1 and M2 was 0.73 ± 0.03, 0.96 ± 0.06 and 0.89 ± 0.03 (n = 6), respectively. The amounts of adrenosterone excreted in urine were 14.75 ng for 48 h. Also, the maximum level of androsterone and 11β-hydroxy androsterone, which were endogenous steroids, were reached 4.1 h after the oral administration of adrenosterone.
Page	1489 - 1496
Full Text	PDF

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