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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 29, Number 9
BKCSDE 29(9)
September 20, 2008 

 
Title
Electron Caption Dissociation of Proteins Initiated by Photoelectrons Generated from 266 nm UV Laser Radiation on an ICR Cell Wall
Author
Sun Young Lee, Soojin Park, Yewon Lee, Han Bin Oh*, Hyuk Kang, Ki Ho Cho, Wha Keun Ahn, Bum Ku Rhee
Keywords
266 nm UV laser, Electron capture dissociation (ECD), Ubiquitin, Azurin, FTICR MS
Abstract
It was demonstrated that ECD MS can be successfully applied to Bovine ubiquitin (8.6 kDa, 76 amino acids) and Pseudomonas aeruginosa azurin (13.9 kDa, 128 amino acids, 1 disulfide bond) cations using photoelectrons emitted by irradiating a 266 nm UV laser light on the edge of an ion cyclotron resonance (ICR) cell trapping plate hole. The photoelectron ECD method cleaved protein backbone bonds as effectively as conventional ECD MS methods that employed a tungsten (W) wire or heated dispenser cathode as an electron source. This method offers the advantage of providing electrons for ECD MS without an apparent electron source.
Page
1673 - 1677
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