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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 29, Number 3
BKCSDE 29(3)
March 20, 2008 

 
Title
Separation of Madecassoside and Madecassic Acid Isomers by High Performance
Liquid Chromatography Using β-Cyclodextrin as Mobile Phase Additive
Author
Guiqing Kai*, Jian Pan, Chuanxun Yuan, Yuan Yuan
Keywords
Madecassoside, Madecassic acid, Isomer, HPLC, Cyclodextrin
Abstract
An improved HPLC method for the separation of madecassoside isomers (madecassoside and asiaticoside-B) has been developed. The isomers can be separated with high resolution from extracts of Centella asiatica by HPLC using β-cyclodextrin as a mobile phase added on a C18 column. The result shows that the isomers can be separated with a mobile phase consisting of methanol:water (50:50, v/v) with 4 mmol/L β-CD. To elucidate the mechanism of the separation of madecassoside and asiaticoside-B, this paper studied the separation of their aglycon parts (madecassic acid and terminolic acid), another pair of isomers. The isomers can also be separated with high resolution with a mobile phase consisting of methanol:water (65:35, v/v) with 4 mmol/L β-CD and the pH of the mobile phase was adjusted to 4. The paper also studied the separation of the two isomers by HPLC using α-CD and Glucosyl-β-CD as a mobile phase additive in order to elucidate the mechanism of the separation process.
Page
551 - 554
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