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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 28, Number 6
BKCSDE 28(6)
June 20, 2007

Title	Molecular Dissection of the Interaction between hBLT2 and the G Protein Alpha Subunits
Author	Krishna Moorthy Vukoti, Won-Kyu Lee, Ho-Jun Kim, Ick Young Kim, Eun Gyeong Yang, Cheolju Lee, Yeon Gyu Yu*
Keywords	Leukotriene B4, hBLT2, G-protein, Intracellular loop, GPCR-G protein interaction
Abstract	Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various Gα protein including Gα i1, Gα i2, Gα i3, Gα s1, Gα o1, and Gα z. Among the tested Gα subunits, Gα i3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (KD) of 5.0 × 10^?6 M. These results suggest that Gα i3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with Gα i3.
Page	1005 - 0
Full Text	PDF

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