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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 28, Number 4
BKCSDE 28(4)
April 20, 2007

Title	Electrochemical Assay of Neurotransmitter Glycine in Brain Cells
Author	Suw Young Ly*, Dong Young Kim
Keywords	Voltammetry, Neurotransmitter, Glycine, Fish, Brain
Abstract	Neurotransmitter glycine in the nano gram range was analyzed using a paste electrode (PE) in cyclic voltammetry (CV) and square-wave stripping voltammetry (SWSV). An anodic peak caused by oxidation of the glycine ion appeared at the 0.4 V (versus Ag/AgCl/KCl) potential in a 0.1 M NH₄H₂PO₄ electrolyte solution. At optimized conditions, the working range of the SWSV and CV concentration was found to be 5-60 ngL^-1 glycine; precision of R² = 0.9816 (SWSV) and 0.9986 (CV); and detection limit of 0.65 ngL^-1 (5.82 × 10^-12 molL^-1) (S/N = 3). The optimized conditions were applied to an assay in a fish brain tissue and a living brain cell in real time.
Page	515 - 519
Full Text	PDF

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