KCS Publications

HOME > Search > Browsing(BKCS) > Archives

Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 26, Number 12
BKCSDE 26(12)
December 20, 2005

Title	Isolation of New Self-Cleaving Ribozymes with in vitro Selection
Author	Bongrae Cho*, Younghoon Lee
Keywords	SELEX, Self-cleavage ribozyme, tRNA, H1 RNA
Abstract	In vitro selection was used to isolate Mg²⁺-dependent self-cleaving ribozymes with cis-cleavage activity from a pre-tRNA library having 40-mer random sequences attached to 5'-end of E. coli tRNA^Phe. After 8 rounds of SELEX (Systematic Evolution of Ligands by Exponential Enrichment), RNA molecules which can self-cleave at the high concentration of Mg²⁺ were isolated. The selected ribozymes can carry out the self-cleavage reaction in the presence of 100 mM Mg²⁺ but not in 10 mM Mg²⁺. The cleavage sites of the ribozymes are located at +3 and +4 of tRNA^Phe, compared with +1 position of 5'-end cleavage site of pre-tRNA by RNase P. New RNA constructs deprived of its D stem-loop, anticodon stem-loop, variable loop and T stem-loop, respectively showed the cleavage specificity identical to a ribozyme having the intact tRNA structure. Also, the new ribozyme fused with both a ribozyme and tRNA^Leu showed the cleavage activities at the various sites within its sequences, different from two sites of position +3 and +4 observed in the ribozyme with tRNA^Phe. Our results suggest that the selected ribozyme is not structural-specific for tRNA.
Page	2033 - 0
Full Text	PDF

How to access to the WOL for The Bulletin of the Korean Chemical Society ?

The Bulletin of the Korean Chemical Society will be co-published with Wiley-VCH from 2015 towards. The online version of the Journal will thus be available on Wiley Online Library, Wiley(-VCH)'s global online platform.

KCS members can get a free online access to the Bulletin of the Korean Chemical Society in WOL.

Free Access to WOL