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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 26, Number 2
BKCSDE 26(2)
February 20, 2005 

Nitrogen Isotope Labeled Tetraheme Cytochrome c3 on a Defined Medium
Andre Kim, Jang-Su Park*
Cytochrome c3, 1H-15N HMQC, Stable isotope
To obtain cytochrome c3 labeled with a stable isotope, the conditions of cultivation and the composition of medium for DvMF were examined. The growth of DvMF was steady and reproducible under purging with N2 and under pH control. DvMF was able to go on a defined medium without natural products. The composition of medium containing a small amount of NH4Cl as sole nitrogen source was established. Then, uniformly 15Nlabeled cytochrome c3 was obtained during the culture of DvMF in a defined medium with 15NH4Cl; it was confirmed by 1H-15N HMQC.
278 - 280
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