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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 25, Number 12
BKCSDE 25(12)
December 20, 2004

Title	Peroxidase Activity of Cytochrome c
Author	Nam Hoon Kim, Moon Sik Jeong, Soo Young Choi, Jung Hoon Kang*
Keywords	Cytochrome c, Peroxidase, Radical scavenger, Iron chelator
Abstract	The peroxidase activity of cytochrome c was studied by using a chromogen, 2,2'-azinobis-(2-ethylbenzthiazoline-6-sulfonate) (ABTS). Initial rate of ABTS oxidation formation was linear with respect to the concentration of cytochrome c between 2.5-10 μM and H2O2 between 0.1-0.5 mM. The optimal pH for the peroxidase activity of cytochrome c was 7.0-8.5. The peroxidase activity retained about 40% of the maximum activity when exposed at 60 oC for 10 min. The peroxidase activity showed a typical Michaelis-Menten kinetics for H2O2 which Km value was 29.6 mM. Radical scavengers inhibited the peroxidase activity of cytochrome c. The peroxidase activity was significantly inhibited by the low concentration of iron chelator, deferoxamine. The results suggested that the peroxidase activity was associated with iron in the heme of cytochrome c.
Page	1889 - 1892
Full Text	PDF

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