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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 22, Number 2
BKCSDE 22(2)
February 20, 2001 

Three Binding Sets Analysis of α-Lactalbumin by Interaction of Tetradecyl Trimethyl Ammonium Bromide
M. R. Housaindokht, J. Chamani, A. A. Saboury, A. A. Moosavi-Movahedi, M. Bahrololoom
TTAB, a-Lactalbumin, Unfolding, Scatchard plot.
The interaction between tetradecyl trimethyl ammonium bromide (TTAB) with bovine α-lactalbumin has been investigated at pH = 9 and at 37℃ by isothermal titration calorimetry, equilibrium dialysis and UV-Vis spectrophotometry methods. The binding data from unusual Scatchard plot have been analyzed in terms of the Hill equation for three sets of binding sites. The calorimetric data show that TTAB interacts endothermically with α-lactalbumin and causes protein unfolding below 2 mM concentration of TTAB, which is confirmed by spectrophotometric data. The unfolding of the protein would be mainly due to occupation of the second set of binding sites.
145 - 148
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