KCS Publications

HOME > Search > Browsing(BKCS) > Archives

Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 21, Number 6
BKCSDE 21(6)
June 20, 2000

Title	Effects of Cysteine on the Inactivation of Bovine Liver Catalase
Author	R. Yousefi, A. A. Saboury, M. Ghadermarzi, A. A. Moosavi-Movahedi
Keywords
Abstract	Bovine liver catalase was exposed to cysteine, as a natural inactivator metabolize, causing autoxidation-generating H2O2 continuously. The catalase species concentrations and activity measurement were done by spectrophotometry in phosphate buffer 10mM, pH 6.5, and 27 ℃. The activity of catalase decreased continuously due to the conversion of active ferricatalase species, E-Fe (III), to an inactive enzyme species, E-Fe (IV). This conversion is related to the slow production of H2O2 generated by autoxidation of cysteine. The free SH-group of cysteine has an essential role in production of H2O2 and hence inactivation of catalase. NADPH can protect catalase against inactivation due to the conversion of inactive form of E-Fe (IV) to ferricatalase species, E-Fe (Ⅲ)
Page	567 - 570
Full Text	PDF

How to access to the WOL for The Bulletin of the Korean Chemical Society ?

The Bulletin of the Korean Chemical Society will be co-published with Wiley-VCH from 2015 towards. The online version of the Journal will thus be available on Wiley Online Library, Wiley(-VCH)'s global online platform.

KCS members can get a free online access to the Bulletin of the Korean Chemical Society in WOL.

Free Access to WOL